Three-dimensional graphic reconstruction of the insect exoskeleton through confocal imaging of endogenous fluorescence

2000 ◽  
Vol 48 (6) ◽  
pp. 367-384 ◽  
Author(s):  
Sasha Zill ◽  
S. Faith Frazier ◽  
David Neff ◽  
Laura Quimby ◽  
Michelle Carney ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Confocal Imaging ◽  
Endogenous Fluorescence

scholarly journals Three-dimensional Super-resolved Imaging of Paraffin-embedded Kidney Samples

2021 ◽  
Author(s):  
David Unnersjoe-Jess ◽  
Amer Ramdedovic ◽  
Martin Hoehne ◽  
Linus Butt ◽  
Felix C Koehler ◽  
...  
Keyword(s):  
New Technologies ◽  
Three Dimensional ◽  
Kidney Tissue ◽  
Confocal Imaging ◽  
Disease Assessment ◽  
Gold Standard Method ◽  
Filtration Barrier ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Monitoring Treatment

Diseases of the glomeruli, the renal filtration units, are a leading cause of progressive kidney disease. Assessment of the ultrastructure of podocytes at the glomerular filtration barrier is essential for diagnosing diverse disease entities, providing insight into the disease pathogenesis as well as monitoring treatment responses. New technologies, including super-resolved nanoscopy and expansion microscopy, as well as new sample preparation techniques, are starting to revolutionize imaging of biopsy specimens. However, our previous approaches for simple and fast three-dimensional imaging of optically cleared samples are to date not compatible with formalin fixed paraffin-embedded (FFPE) tissue, impeding application in clinical routine. Here we provide protocols that circumvent these limitations and allow for three dimensional STED and confocal imaging of FFPE kidney tissue with similar staining and image quality as compared to our previous approaches. This would increase the feasibility to implement these protocols in clinical routines, as FFPE is the gold standard method for storage of patient samples.

scholarly journals The Influence of Astaxanthin on the Proliferation of Adipose-derived Mesenchymal Stem Cells in Gelatin-Methacryloyl (GelMA) Hydrogels

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2416 ◽  
Author(s):  
Bo Young Choi ◽  
Elna Paul Chalisserry ◽  
Myoung Hwan Kim ◽  
Hyun Wook Kang ◽  
Il-Whan Choi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cellular Proliferation ◽  
Three Dimensional ◽  
Confocal Imaging ◽  
Alamar Blue Assay ◽  
Ratio Test ◽  
Alamar Blue ◽  
Chemical Structures

Recently, astaxanthin, a red lipophilic pigment belonging to the xanthophyllic family of carotenoids, has shown the feasibility of its uses in tissue engineering and regenerative medicine, due to its excellent antioxidant activities and its abilities to enhance the self-renewal potency of stem cells. In this study, we demonstrate the influence of astaxanthin on the proliferation of adipose-derived mesenchymal stem cells in tissue-engineered constructs. The tissue engineered scaffolds were fabricated using photopolymerizable gelatin methacryloyl (GelMA) with different concentrations of astaxanthin. The effects of astaxanthin on cellular proliferation in two-dimensional environments were assessed using alamar blue assay and reverse transcription polymerase chain reaction (RT-PCR). Then, rheological properties, chemical structures and the water absorption of the fabricated astaxanthin-incorporated GelMA hydrogels were characterized using NMR analysis, rheological analysis and a swelling ratio test. Finally, the influence in three-dimensional environments of astaxanthin-incorporated GelMA hydrogels on the proliferative potentials of adipose-derived stem cells was assessed using alamar blue assay and the confocal imaging with Live/dead staining. The experimental results of the study indicate that an addition of astaxanthin promises to induce stem cell potency via proliferation, and that it can be a useful tool for a three-dimensional culture system and various tissue engineering applications.

scholarly journals Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity

2014 ◽  
Vol 306 (3) ◽  
pp. E233-E246 ◽  
Author(s):  
Denise E. Lackey ◽  
David H. Burk ◽  
Mohamed R. Ali ◽  
Rouzbeh Mostaedi ◽  
William H. Smith ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Adipose Tissue ◽  
Proteolytic Enzymes ◽  
Three Dimensional ◽  
Confocal Imaging ◽  
Obese Women ◽  
Multivariate Statistical ◽  
Related Factors ◽  
Ecm Remodeling ◽  
Stress Signals

The extracellular matrix (ECM) plays an important role in the maintenance of white adipose tissue (WAT) architecture and function, and proper ECM remodeling is critical to support WAT malleability to accomodate changes in energy storage needs. Obesity and adipocyte hypertrophy place a strain on the ECM remodeling machinery, which may promote disordered ECM and altered tissue integrity and could promote proinflammatory and cell stress signals. To explore these questions, new methods were developed to quantify omental and subcutaneous WAT tensile strength and WAT collagen content by three-dimensional confocal imaging, using collagen VI knockout mice as a methods validation tool. These methods, combined with comprehensive measurement of WAT ECM proteolytic enzymes, transcript, and blood analyte analyses, were used to identify unique pathophenotypes of metabolic syndrome and type 2 diabetes mellitus in obese women, using multivariate statistical modeling and univariate comparisons with weight-matched healthy obese individuals. In addition to the expected differences in inflammation and glycemic control, approximately 20 ECM-related factors, including omental tensile strength, collagen, and enzyme transcripts, helped discriminate metabolically compromised obesity. This is consistent with the hypothesis that WAT ECM physiology is intimately linked to metabolic health in obese humans, and the studies provide new tools to explore this relationship.

Three-dimensional confocal imaging of objects embedded within thick diffusing media

1991 ◽  
Vol 30 (14) ◽  
pp. 1796 ◽  
Author(s):  
David S. Dilworth ◽  
Emmett N. Leith ◽  
Joaquin L. Lopez
Keyword(s):  
Three Dimensional ◽  
Confocal Imaging

scholarly journals Noninvasive Pigment Identification in Single Cells from Living Phototrophic Biofilms by Confocal Imaging Spectrofluorometry

2004 ◽  
Vol 70 (6) ◽  
pp. 3745-3750 ◽  
Author(s):  
M. Rold�n ◽  
F. Thomas ◽  
S. Castel ◽  
A. Quesada ◽  
M. Hern�ndez-Marin�
Keyword(s):  
Single Cells ◽  
Three Dimensional ◽  
Confocal Imaging ◽  
Confocal Scanning Laser Microscopy ◽  
Pigment Analysis ◽  
Phototrophic Biofilms ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

ABSTRACT A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.

scholarly journals Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice

2008 ◽  
Vol 295 (4) ◽  
pp. F1142-F1148 ◽  
Author(s):  
Fannie Darlot ◽  
Annie Artuso ◽  
Nicole Lautredou-Audouy ◽  
Daniel Casellas
Keyword(s):  
Schwann Cells ◽  
Sympathetic Innervation ◽  
Three Dimensional ◽  
Cell Types ◽  
Confocal Imaging ◽  
Egfp Expression ◽  
Cell Processes ◽  
Fluorescence Confocal Imaging ◽  
Afferent Arterioles ◽  
Protein S100b

Schwann cells (Sc), associated axons, and nearby vascular endothelium constitute a functional trilogy of major importance during the development and regrowth of peripheral vascular nerves. The goal of the present study is to provide a technique of triple fluorescence confocal imaging of these cell types along renal preglomerular vessels. We took advantage of a protein S100B/EGFP transgenic mouse to visualize Sc. The endothelium was labeled with an intravenous injection of fluorescently tagged lectin, and after tissue processing, adrenergic nerves were revealed with an antibody against the marker protein synaptophysin. As a validation step, we found that EGFP-positive perivascular cells with prominent cell bodies and extensive, multidirectional cell processes were protein S100B positive. They were identified as Sc and indirectly assumed to be unmyelinated Sc. By contrast, we found strong EGFP expression in proximal epithelial cells and in the epithelium lining thin limbs of Henle. This epithelial fluorescence was not associated with immunoreactive protein S100B and thus corresponded to ectopic EGFP expressions in this mouse strain. Sc were organized in bundles or as a meshwork surrounding the preglomerular vasculature from arcuate arteries to afferent arterioles. No Sc were detected in the medulla. Although most Sc were closely apposed to adrenergic varicosities, many varicosities were not associated with detectable Sc processes. The present technique, and the capacity of confocal microscopy to yield three-dimensional imaging, allow the study of the microtopology of Sc and related sympathetic axons in the renal perivascular interstitium.

Sign in / Sign up

Export Citation Format

Share Document